人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pDMS197
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
pDMS197
pDMS197
規格:
貨期:
編號:B161742
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pDMS197
商品貨號 B161742
Designations pDMS197
Depositors DM Schifferli
Biosafety Level 1
Host Escherichia coli SM10 lambda pir
Vector Information
Size (kb): 6.50
Type of vector: plasmid
Cloning sites: BamHI XbaI KpnI SacI SmaI
Polylinker sites: BamHI HindIII XbaI KpnI SphI EcoRV SacI SmaI EcoRI
Vector: pDMS197 (plasmid)
Construction: pRE107, tetR (pBR322)
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
MCS: BamHI...EcoRI, ->
marker(s): sacB1, ->
restriction site: EcoRI HindIII, ->
replicon: conditional R6K ori
replicon: oriT
marker(s): tetR, ->
Applications Counterselection levansucrase
Suicide vector
Vector permitting allelic exchange of cloned inserts
Comments Restriction digests of the clone give the following sizes (kb): XbaI-- 6.55; KpnI-- 6.55; HindIII-- 2.9, 2.2, 1.4.

The sacB1 allele is a variant of sacB with certain restriction sites removed by site directed mutagenesis. Expression of sacB confers sensitivity to sucrose.

The conditional R6K origin of replication requires that the pi protein be expressed in trans for plasmid maintenance.

One of four allelic exchange suicide vectors (ATCC 87691 - 87694) that provide both selection for chromosomal integration (ampR, cmlR, kanR or tetR) and counterselection for loss of vector DNA and the wild type allele.

Construct is suicide plasmid in any host not expressing pir.

Cloned inserts may be integrated into the host chromosome (a single recombination event) following electroporation and appropriate antibiotic selection.

Negative selection for sucrose sensitivity (sacB) selects for a second recombination event resulting in loss of vector DNA.
Media ATCC® Medium 1631: LB medium (ATCC medium 1065) with 10 mcg/ml tetracycline
Growth Conditions
Temperature: 37°C
References

Edwards RA, et al. Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression. Gene 207: 149-157, 1998. PubMed: 9511756

Donnenberg MS, Kaper JB. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59: 4310-4317, 1991. PubMed: 1937792

Blomfield IC, et al. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 5: 1447-1457, 1991. PubMed: 1686293

Kolter R, et al. Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K. Cell 15: 1199-1208, 1978. PubMed: 728998

McIver KS, et al. The elastase propeptide functions as an intramolecular chaperone required for elastase activity and secretion in Pseudomonas aeruginosa. Mol. Microbiol. 18: 877-889, 1995. PubMed: 8825092

Shipped frozen
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板： 天天av天天翘天天综合网色鬼| 久热这里只有精品99在线观看| yyyy11111少妇影院| 国产性夜夜春夜夜爽免费下载| 成人毛片18女人毛片免费| 一本大道无码人妻精品专区 | 好男人社区神马在线观看www| 国产成人精品视频网站| 亚洲天天做日日做天天欢毛片| 无码无套少妇毛多69xxx| 国产爆乳无码视频在线观看3| 欧美一区二区三区性视频| 男女做爰猛烈啪啪吃奶伸舌头下载| 欧美人与牲动交xxxx| 亚洲国产成人久久精品大牛影视| 宅男66lu国产在线观看| 中文字幕无码中文字幕有码a| 苍井空一区二区波多野结衣av| 国产精品久久久久久妇女| 久久中文字幕无码a片不卡古代| 欧美 日韩 国产 另类 图片区| 性xxxx视频播放免费| 国产男女无遮挡猛进猛出 | 加勒比一本heyzo高清视频| 亚洲人成电影网站色迅雷| 久久一卡二卡三卡四卡| 国产伦理一区二区| 一本久久综合亚洲鲁鲁五月天| 西西艺术人像摄影| 粉嫩小泬无遮挡久久久久久| 亚洲日韩av一区二区三区四区| 亚洲国产精品嫩草影院永久| 亚洲精品色情app在线下载观看| 亚洲精品蜜夜内射| 一线二线无人区乱码| 亚洲一线二线三线品牌精华液久久久 | 成人欧美一区二区三区在线观看| 四虎亚洲精品高清在线观看| 欧洲精品一线二线三线区别| 无码人妻精品一区二区在线视频| 无码av动漫精品一区二区免费 |