| 產品名稱 |
17aba |
| 商品貨號 |
B162227 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Applications |
The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro. The antibody cross-reacts with CD11b from rat and canine cells. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD11b |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD11b |
| Comments |
Animals were immunized with human acute monocytic leukemia cells (FAM M6). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with human CD11b, and blocks all known CD11b dependent activities in vitro. The antibody cross-reacts with CD11b from rat and canine cells. |
| Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below), 90%; Gamma
globulin-free horse serum, 10%.
DME medium with 4.5 g/L glucose 100 mL
L-Glutamine (100x) 2 mL
Solution I 1 mL
Hypoxanthine (10 mM) - Thymidine (1.6 mM) 1 mL
NCTC 135 10 mL
Gamma Globulin-Free Horse Serum (inactivated) 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM; kappa light chain |
| Name of Depositor |
RF Todd |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
. . J. Inflamm. 13: 495-505, 1989.
Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017
Mulligan MS, et al. Roles of beta 2 integrins of rat neutrophils in complement- and oxygen radical-mediated acute inflammatory injury. J. Immunol. 148: 1847-1857, 1992. PubMed: 1347308
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