Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.0; BamHI--7.0; HindIII--5.0, 2.0; SalI--7.0; HindIII/PvuII--3.2, 2.6, 2.0. Corresponds to nt 8545 to 9526 of the sequence record (exon 2 extends from 9121 through 9858) Includes EcoRI fragment from intron 1 through part of precursor sequences of beta-NGF into coding region of active beta-NGF. |
