Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
ATCC® VR-1479™ (M-varient) is a cell culture passaged linage of the M-varient (ATCC® VR-1314™) reported to induce diabetes in selected strains of mice such as Balb/cBy and DBA/2 mice. Note that virus loses diabetagenic properties upon multiple passage in cell culture.
Effect on Host
Rounding and sloughing with general degeneration
Recommended Host
NCTC Clone 929 (ATCC® CCL-1™)
Growth Conditions
Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 75-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1 hour at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days
Name of Depositor
ATCC
Special Collection
NCRR Contract
Source
ATCC® VR-1479™ was created by serially passaging ATCC® VR-1314™ two times onto NCTC clone 929 (L-929) cells, ATCC® CCL-1™. The original strain was isolated from the heart of mice inoculated by peripheral route with EMC of swine.
References
Craighead JE. Pathogenicity of the M and E variants of the encephalomyocarditis (EMC) virus. I. Myocardiotropic and neurotropic properties. Am. J. Pathol. 48: 333-345, 1966. PubMed: 4285783
