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Felid herpesvirus 1
Felid herpesvirus 1
規格:
貨期:
編號:B163249
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Felid herpesvirus 1
商品貨號 B163249
Classification Herpesviridae, Varicellovirus
Deposited As Feline rhinotracheitis virus
Agent Felid herpesvirus 1
Strain FVRm (modified)
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images ATCC VR-1787 images
Comments Derived by adaptation from primary cell culture product ATCC® VR-815™ to CRFK cells (ATCC® CCL-94™).  ATCC® VR-815™ and ATCC® VR-1787™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. Item is a vaccine strain. Antigenicity is specific for virus.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
CRFK (ATCC® CCL-94™)
Growth Conditions Temperature: 31°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 31°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-5 days
Name of Depositor EV Davis, Norden Laboratories, Inc.
Source Throat swab from a 3 month old cat
References

Davis E. Feline viral rhinotracheitis vaccine and combination feline viral rhinotracheitis-calicivirus vaccine prepared therefrom. US Patent 4,031,204 dated Jun 21 1977

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