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Vaccinia virus ts mutant
Vaccinia virus ts mutant
規格:
貨期:
編號:B163264
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
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產品名稱 Vaccinia virus ts mutant
商品貨號 B163264
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts77 (Dales isolate 8933)
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U17.
Positive for Mycoplasma No
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder
Comments
Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM categories A and K indicating absence of mature virus structures, and presence of granular foci with immature particles containing nuceoids but no internal dense material. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U17.
Effect on Host
Yes, Cytopathic effect: Rounding and sloughing of infected cells
CPE: enlargement, plaques with rounding and eventual sloughing
Recommended Host

Recommended Host: BSC-40 (ATCC CRL-2761
Growth Conditions
Temperature: 31.0°C
Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Name of Depositor S Dales, RC Condit, S Dales
Special Collection NCRR Contract
Source
derived from existing strain
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain.

cell enlargement, plaques with rounding and eventual sloughing of cells

Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

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