| 產品名稱 |
19F8 |
| 商品貨號 |
B163611 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
mixed: adherent and suspension, The cells are rather adherent in nature, and grow best if maintained in suspension culture plates (e.g., Corning Catalog number 430591). |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Wistar (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with CD1.1, and cross-reacts with CD1.2. The antibody performs well in flow cytometry applications, but weakly in immunoprecipitation applications. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse CD1.1 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse CD1.1 |
| Comments |
Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected). Spleen cells were fused with Sp2/0-Ag14 cells. The antibody does not block recognition of CD1.1 by T cells. The antibody reacts with CD1.1, and cross-reacts with CD1.2. The epitope recognized by 19F8 is distinct from that recognized by the cluster represented by 15F7 (ATCC HB-322) and 20H2 (ATCC HB-323). The antibody performs well in flow cytometry applications, but weakly in immunoprecipitation applications. |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 95%; fetal bovine serum, 5%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Maintain cultures at cell concentrations between 4 X 10(5) and 8 X 10(5) viable cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2b; kappa light chain |
| Name of Depositor |
A Bendelac |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Roark JH, et al. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127, 1998. PubMed: 9531266
Park SH, et al. Tissue-specific recognition of mouse CD1 molecules. J. Immunol. 160: 3128-3134, 1998. PubMed: 9531267
Park SH, et al. Innate and adaptive functions of the CD1 pathway of antigen presentation. Semin. Immunol. 10: 391-398, 1998. PubMed: 9799714
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