| Comments |
Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida. The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990. |
| References |
Martineau-Doize B, et al. Effects of purified Pasteurella multocida dermonecrotoxin on cartilage and bone of the nasal ventral conchae of the piglet. Anat. Rec. 228: 237-246, 1990. PubMed: 2260779
Magyar T, Rimler RB. Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay. J. Clin. Microbiol. 29: 1328-1332, 1991. PubMed: 1885729
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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