| 產品名稱 |
200-3-G6-4 (20.4) |
| 商品貨號 |
B163633 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to the 75000 dalton NGF receptor of primates. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates |
| Cellular Products |
immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with human melanoma cell lines. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody binds to the 75000 dalton NGF receptor of primates. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: every 2 to 3 days |
| Cryopreservation |
Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
Wistar Institute |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Koprowski H, et al. Diagnostic method for detection of neural crest disease. US Patent 4,786,593 dated Nov 22 1988
Johnson EM Jr.. Tumor diagnostic method. US Patent 4,855,241 dated Aug 8 1989
Levi AD, et al. The functional characteristics of Schwann cells cultured from human peripheral nerve after transplantation into a gap within the rat sciatic nerve. J. Neurosci. 14: 1309-1319, 1994. PubMed: 8120626
|
