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Animals were immunized with a synthetic polypeptide with the sequence LMCQCWRKDPEERPTF corresponding to peptides 494 to 509 of the v-src oncogene (formerly 273 to 284).

In ELISA testing, the antibody cross reacts with the homologous region of FES, v-mos, erb B, v-src, FGR, yes, mil, FMS, abl, ros, lck, and MET. Weak activity was detected for PKS and fps.

Binding was not detected for c-mos, MOS, SRC, ros, rel, FMS, HIR, TRK, sea, and kit.

Spleen cells were fused with Sp2/0 myeloma cells.

immunoglobulin; monoclonal antibody; against a v-src synthetic oncogene peptide

immunoglobulin; monoclonal antibody; against a v-src synthetic oncogene peptide

Animals were immunized with a synthetic polypeptide with the sequence LMCQCWRKDPEERPTF corresponding to peptides 494 to 509 of the v-src oncogene (formerly 273 to 284).

Spleen cells were fused with Sp2/0 myeloma cells.

In ELISA testing, the antibody cross reacts with the homologous region of FES, v-mos, erb B, v-src, FGR, yes, mil, FMS, abl, ros, lck, and MET. Weak activity was detected for PKS and fps.

Binding was not detected for c-mos, MOS, SRC, ros, rel, FMS, HIR, TRK, sea, and kit. In immunoblot testing, a protein of approximately 40 kd is detected in fetal brain.

The IgG1 reactivity of the antibody with the v-src peptide (494 to 509) was confirmed by ELISA.

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

culture medium 95%; DMSO, 5%

Temperature: 37.0°C