| 產品名稱 |
2E6 |
| 商品貨號 |
B163683 |
| Organism |
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type |
hybridoma:lymphoblast B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes. |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse beta 2 leukocyte integrins (CD18) |
| Comments |
Animals were immunized with mouse dendritic cells. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-225) from murine leukocytes. 2E6 blocks the binding function of LFA-1 (CD11a) and CR3/Mac-1 (CD11b). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG |
| Name of Depositor |
RM Steinman |
| Deposited As |
hamster, Armenian (B cell); mouse (myeloma) |
| References |
Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332
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