| 產品名稱 |
32B11 |
| 商品貨號 |
B163703 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with glomalin on the surface of hyphae and spores and also on glomalin sloughed from hyphae. Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. The antibody is used for studies on soil structure; it is useful in ELISA assays to detect glomalin on the surface of hyphae and glomalin in root cortical cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. It is found worldwide and it may the primary glue that holds soil together. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against glomalin (a glycoprotein in soil) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against glomalin (a glycoprotein in soil) |
| Comments |
Animals were immunized with freshly collected Glomus intraradices FL208 spores. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody reacts with glomalin on the surface of hyphae and spores and also on glomalin sloughed from hyphae. The antibody is slightly cross-reactive with Leptospharia korrea. Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. It is found worldwide and it may the primary glue that holds soil together. The antibody is used for studies on soil structure; it is useful in ELISA assays to detect glomalin on the surface of hyphae and glomalin in root cortical cells. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
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| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Attached cells may be subcultured by scraping. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: culture medium 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM; kappa light chain |
| Name of Depositor |
SF Wright |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Wright SF, et al. Time-course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during active colonization of roots. Plant Soil 181: 193-203, 1996.
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