| 產(chǎn)品名稱 |
48 |
| 商品貨號(hào) |
B163756 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Erythroleukemia |
| Applications |
The antibody is highly specific for the gp70 of FrMuLV. The antibody efficiently neutralizes FrMuLV and immunoprecipitates gp70 from infected cell lysates, but reacts poorly with fixed cells and Western blots. |
| Derivation |
Spleen cells from a mouse recovering from erythroleukemia induced by infection with B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against gp70 envelop antigen (ENV) protein of murine leukemia viruses (MuLV) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against gp70 envelop antigen (ENV) protein of murine leukemia viruses (MuLV) |
| Comments |
Spleen cells from a mouse recovering from erythroleukemia induced by infection with B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells. The antibody is highly specific for the gp70 of FrMuLV. The antibody efficiently neutralizes FrMuLV and immunoprecipitates gp70 from infected cell lysates, but reacts poorly with fixed cells and Western blots. The antibody can distinguish FrMuLV and Rauscher MuLV (RaMuLV) from other types of MuLV. |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
B Chesebro |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798
Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
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