| 產品名稱 |
4C5G |
| 商品貨號 |
B163762 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with type II PtdIns 4-kinase from bovine brain. The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity. |
| Derivation |
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain. Lymph node cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain |
| Comments |
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain. Lymph node cells were fused with Sp2/0 mouse myeloma cells. The antibody reacts with type II PtdIns 4-kinase from bovine brain. The antibody causes greater than 90% inhibition of type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. It causes 46% inhibition of type II PtdIns 4-kinase from Xenopus laevis oocytes. It fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver. The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
G Endemann |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Endemann GC, et al. A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase. Biochem. J. 273: 63-66, 1991. PubMed: 1846531
Del Vecchio RL, Pilch PF. Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. J. Biol. Chem. 266: 13278-13283, 1991. PubMed: 1649187
De Neef RS, et al. Relationship between type II phosphatidylinositol 4-kinase activity and protein tyrosine phosphorylation in membranes from normal and sickle red cells. Eur. J. Biochem. 235: 549-556, 1996. PubMed: 8654400
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