| 產品名稱 |
4E8C12 |
| 商品貨號 |
B163769 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons. |
| Complete Growth Medium |
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Isotype |
Secretes a mouse IgG2a monoclonal antibody specific for enteropathogenic
Escherichia coli serotypes O157:H7 and O26:H11. |
| Name of Depositor |
Wisconsin Alumni Res. Fndn. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992
Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994
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