| 產(chǎn)品名稱(chēng) |
AS 33 |
| 商品貨號(hào) |
B163958 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with some colon and pancreatic carcinoma cell lines as well as some T cell leukemia lines. Some reactivity was also detected in salivary gland and breast tissue. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a heat stable human carcinoma associated antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a heat stable human carcinoma associated antigen |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in nude mice |
| Comments |
Animals were immunized with the ASPC-1 human pancreatic carcinoma cell line. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with some colon and pancreatic carcinoma cell lines as well as some T cell leukemia lines. In tissues, the reactivity is restricted to carcinomas and normal colonic mucosa. Some reactivity was also detected in salivary gland and breast tissue. Other normal tissues test were negative. The AS 33 antigen is heat stable. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Isotype |
IgG2a , IgG2a |
| Name of Depositor |
Sloan-Kettering Institute for Cancer Research |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Welt S, et al. Method of imaging colorectal carcinoma lesion and composition for use therein. US Patent 5,160,723 dated Nov 3 1992
Welt S, et al. Methods of treating colon cancer utilizing tumor-specific antibodies. US Patent 5,851,526 dated Dec 22 1998
|
