| 產(chǎn)品名稱 |
c37 (B7IFi1) |
| 商品貨號 |
B164086 |
| Organism |
Mus musculus, mouse |
| Tissue |
liver |
| Cell Type |
epithelial |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hepatoma |
| Strain |
C57L/J |
| Applications |
ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme. |
| Comments |
The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme. |
| Complete Growth Medium |
Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing |
Protocol: - Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor |
O Hankinson |
| Deposited As |
mouse |
| References |
Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390
Kimura S, et al. Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast. EMBO J. 6: 1929-1933, 1987. PubMed: 3308449
The c37 (B7IFi1) cell line was derived from Hepa-1c1c7 (ATCC CRL-2026). Hepa-1c1c7 has high aryl hydrocarbon hydroxylase (AHH) activity. ICR191G (a frame shift mutagen) was used to treat Hepa-1c1c7 cells. Mutated colonies were selected for benzo[a]pyrene resistance. The c37 (B7IFi1) cell line is one of these, and lacks cytochrome P4501A1 dependent aryl hydrocarbon hydroylase (AHH) activity due to two point mutations in the CYP1A1 protein (Leu-118 to Arg and Arg-245 to Pro). The line may be used to study xenobiotic (and carcinogenic) metabolism in the absence of cytochrome P4501A1 activity, which is known to metabolize benzo[a]pyrene cytotoxic and carcinogenic intermediates. It is also a tool to study the putative natural ligand for the induction of this enzyme.
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