| 產(chǎn)品名稱 |
Cates-1B |
| 商品貨號 |
B164122 |
| Organism |
Homo sapiens, human |
| Tissue |
testis; Derived from metastatic site: lymph node |
| Product Format |
frozen |
| Morphology |
mixed |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
embryonal carcinoma |
| Age |
34 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 46; range = 40 to 53.
This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 56% of cells. The rate of polyploidy was 1.9%. There were no constitutive marker chromosomes. The N2 was trisomic. There was a high incidence of Y chromosome losses, which contributed to the high number of cells with 46 chromosomes. |
| Clinical Data |
male Caucasian 34 years |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing |
Protocol: - Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Interval: Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| STR Profile |
Amelogenin: XY CSF1PO: 10 D13S317: 12 D16S539: 12, 13 D5S818: 12, 13 D7S820: 7, 12 THO1: 6 TPOX: 8 vWA: 16, 17 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
J Fogh |
| Deposited As |
Homo sapiens |
| Year of Origin |
1973 |
| References |
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
|
