| 產(chǎn)品名稱 |
CC38 |
| 商品貨號(hào) |
B164132 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the bovine CD6 antigen (primarily expressed on mature circulating CD2 positive T cells). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine CD6 (BoCD6) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine CD6 (BoCD6) |
| Comments |
Animals were immunized with bovine lymphocytes and thymocytes. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for the bovine CD6 antigen (primarily expressed on mature circulating CD2 positive T cells). This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG2b |
| Name of Depositor |
CJ Howard |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Seidler RJ, et al. L-selectin expression differentiates T cells isolated from different lymphoid tissues in cattle but does not correlate with memory. Immunology 77: 228-234, 1992. PubMed: 1385313
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