| 產品名稱 | DB |
|---|---|
| 商品貨號 | B164334 |
| Organism | Homo sapiens, human |
| Tissue | Ascites |
| Cell Type | B lymphoblast |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | large cell lymphoma |
| Age | 45 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma. |
| Clinical Data | 45 years
Caucasian
male
|
| Antigen Expression | CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 - |
| Cellular Products | immunoglobulin |
| Comments | The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor.
Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition.
They are reported to be negative for the Epstein-Barr virus genome.
The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL. Medium Renewal: 2 to 3 times weekly. |
| Cryopreservation | Freeze Medium: Complete growth medium, 90%; DMSO, 10%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11,12 D16S539: 11 D5S818: 11 D7S820: 11 THO1: 6,8 TPOX: 8 vWA: 15,16 |
| Isotype | IgG; lambda light chain |
| Name of Depositor | M Beckwith |
| Deposited As | Homo sapiens |
| References | Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
