| 產(chǎn)品名稱 |
FOX-NY |
| 商品貨號 |
B164467 |
| Organism |
Mus musculus, mouse |
| Cell Type |
B lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
myeloma |
| Strain |
BALB/c |
| Applications |
FOX-NY was derived from the NS-1 myeloma cell line. It is resistant to analogs of both adenosine and guanine (e. When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus. Selection for the Igh locus results in more stable antibody production in the resulting hybridomas. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
FOX-NY was derived from the NS-1 myeloma cell line. |
| Clinical Data |
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus. |
| Comments |
FOX-NY was derived from the NS-1 myeloma cell line. It is resistant to analogs of both adenosine and guanine (e.g., 2,6-diaminopurine and 8-azaguanine) as a result of deficiencies in hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) and adenosine phosphoribosyl transferase (APRT). When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus. If the fusion products are grown in the presence of aminopterin, adenine and thymidine (or azaserine and adenine). Selection for the Igh locus results in more stable antibody production in the resulting hybridomas. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor |
RT Taggart |
| Deposited As |
Mus musculus |
| References |
Taggart RT, Samloff IM. Stable antibody-producing murine hybridomas. Science 219: 1228-1230, 1983. PubMed: 6402815
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