| 產品名稱 |
G-361 |
| 商品貨號 |
B164482 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Product Format |
frozen |
| Morphology |
epithelial |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
malignant melanoma |
| Age |
31 years adult |
| Gender |
male |
| Ethnicity |
Caucasian |
| Clinical Data |
31 years adult Caucasian male |
| Genes Expressed |
melanin |
| Cellular Products |
melanin |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 15 D5S818: 11 D7S820: 8,11 THO1: 7,9 TPOX: 8,10 vWA: 15,17 |
| Isoenzymes |
G6PD, B |
| Name of Depositor |
PT Peebles |
| Deposited As |
Homo sapiens |
| References |
Peebles P, et al. Isolation of four unusual pediatric solid tumor cell lines. Pediatr. Res. 12: 485, 1978.
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