人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Hs 606.T
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
Hs 606.T
Hs 606.T
規格:
貨期:
編號:B164702
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Hs 606.T
商品貨號 B164702
Organism Homo sapiens, human
Tissue mammary gland; breast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease carcinoma
Age 44 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images
Clinical Data
female
Caucasian
44 years
Comments
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37.0°C .
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: complete growth medium, 90%; DMSO, 10%
liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 11,12
D5S818: 11,13
D7S820: 8,10
THO1: 7,9
TPOX: 8
vWA: 14,19
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 欧美激情一区二区| 国产一区二区三区内射高清| www一区二区www免费| 欧美性xxxx极品少妇| 永久黄网站色视频免费无下载| 久久久无码精品国产一区| 九九综合va免费看| 图片小说视频一区二区| 久久免费精品国产72精品| 午夜福利午夜福利1000| 一区一区三区产品乱码| 日本体内she精高潮| 青草青草久热国产精品| 久久久久久亚洲精品a片成人| 长腿校花无力呻吟娇喘的视频| 国产成人精品无码片区在线观看| 精品亚洲欧美自拍| 日本无码一区二区三区不卡免费| 久久久久久av无码免费看大片 | 蜜色欲多人av久久无码| 嫩草欧美曰韩国产大片| 欧美肥老太牲交大战| 无码粉嫩虎白一线天在线观看| 国产大屁股视频免费区| 变态另类牲交乱| 精品无码国产一区二区三区av| 初尝人妻少妇中文字幕| 婷婷五月日韩av永久免费| 亚洲卡一卡二新区| 欧美性白人极品hd| 丰满少妇人妻久久久久久| 99久久无色码中文字幕人妻| 亚洲自偷自拍另类11p| 久久久久久自慰出白浆| 亚洲国产精品高清久久久| 久久精品www人人做人人爽 | 午夜亚洲精品久久一区二区| 麻豆亚洲一区| 337p人体 欧洲人体 亚洲| 亚洲成av人片在线观l看福利1| 麻豆一区二区三区蜜桃免费|