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This cell line is a suitable transfection host.

The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation.

13 months

Caucasian

male

Two cell types are present. Predominant is a small neuroblast-like cell.The other is a large hyaline fibroblast.

Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
5. Add appropriate aliquots of the cell suspension to new culture vessels. Maintain cultures at a cell concentration between 4x10⁴ and 4 x 10⁵ cells/cm².
6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Temperature: 37°C

Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 9

D16S539: 8

D5S818: 11,12

D7S820: 9,10

THO1: 7,9.3

TPOX: 11

vWA: 15

G6PD, B

The cell line was submitted to the American Type Culture Collection in the 36th passage. It has been demonstrated that the cells can be propagated successfully beyond the 80th serial subculture.