| 產品名稱 |
IVD12 |
| 商品貨號 |
B164865 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody identifies the HLA DQw3 supertypic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA DQ |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA DQ |
| Comments |
Animals were immunized with the PRIESS human B cell line. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody identifies the HLA DQw3 supertypic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and
1 mM sodium pyruvate, 85%; fetal bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
JD Capra, RC Giles, CK Hurley |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Giles RC, et al. Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity. J. Exp. Med. 157: 1461-1470, 1983. PubMed: 6189938
|
