| 產(chǎn)品名稱 |
KMI6 |
| 商品貨號(hào) |
B164925 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antigenic specificity has now been identified as the murine integrin beta 1 homolog. Unlike the alpha 4 specific antibody PS/2 (see ATCC CRL-1911), KMI6 did not block lymphoid cell adhesion. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse integrin beta 1 subunit (CD29) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse integrin beta 1 subunit (CD29) |
| Comments |
Animals were immunized with BMS2 murine bone marrow stromal cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. This line was originally characterized as a murine stromal cell restricted marker. The antigenic specificity has now been identified as the murine integrin beta 1 homolog. Unlike the alpha 4 specific antibody PS/2 (see ATCC CRL-1911), KMI6 did not block lymphoid cell adhesion. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
PW Kincade |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Wu X, et al. Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody. Hybridoma 13: 409-416, 1994. PubMed: 7532155
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