| 產品名稱 |
L-14 [L-14-SCRF 55.1] |
| 商品貨號 |
B164936 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 72000 dalton HMCV immediate - early perinuclear antigen. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human cytomegalovirus (HMCV) immediate - early 72000 dalton antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human cytomegalovirus (HMCV) immediate - early 72000 dalton antigen |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites forms ascites in BALB/c mice |
| Comments |
Animals were immunized with CMV infected human fibroblasts. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a 72000 dalton HMCV immediate - early perinuclear antigen. The antigen is expressed within three hours of infection with HCMV. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml. |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Isotype |
IgG1 |
| Name of Depositor |
Scripps Clinic and Research Foundation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Rice GP, et al. Cytomegalovirus infects human lymphocytes and monocytes: Virus expression is restricted to immediate-early gene products. Proc. Natl. Acad. Sci. USA 81: 6134-6138, 1984. PubMed: 6091137
March CJ, et al. Hybridoma antibody which binds IL-2 receptor. US Patent 4,845,198 dated Jul 4 1989
March CJ, et al. Interleukin-2 receptor. US Patent 4,578,335 dated Mar 25 1986
Oldstone MB, Rice G. Diagnostic system for the detection of cytomegalovirus. US Patent 4,818,678 dated Apr 4 1989
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