| 產品名稱 |
MS1 (MILE SVEN 1) |
| 商品貨號 |
B165196 |
| Organism |
Mus musculus, mouse |
| Tissue |
pancreas/islet of Langerhans; endothelium |
| Cell Type |
SV40 transformed |
| Product Format |
frozen |
| Morphology |
endothelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 [Cells contain polyomavirus DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
C57BL/6 |
| Applications |
This cell line is useful for the study of signal transduction of angiogenic factors.
This cell line is the only line known that can give rise to benign hemangiomas.
|
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
MS1 is a pancreatic islet endothelial cell line established in 1994. Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418. Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL. |
| Receptor Expression |
vascular endothelial growth factor (VEGF), expressed |
| Genes Expressed |
tissue inhibitor of bioreactive matrix metalloproteinase (high levels) |
| Cellular Products |
tissue inhibitor of bioreactive matrix metalloproteinase (high levels) |
| Comments |
The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.
The expression of high levels of the tissue inhibitor of bioreactive matrix metalloproteinase makes the behavior of this cell line more like that of normal macrophages from some commonly used strains of mice. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subculture Ratio: 1:4 to 1:8
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
JL Arbiser |
| Deposited As |
Mus musculus |
| Year of Origin |
1994 |
| References |
Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347
Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370
|
