| 產品名稱 |
N1-S1 |
| 商品貨號 |
B165218 |
| Organism |
Rattus norvegicus, rat |
| Tissue |
liver |
| Product Format |
frozen |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Hepatoma; Novikoff hepatoma |
| Gender |
male |
| Strain |
Sprague-Dawley |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The N1-S1 cell line was established from a hepatoma induced by feeding 4-dimethylaminoazobenzene to a male rat. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 104 viable cells/mL and subculture at 1 to 1.5 X 106 cells/mL (after about 3 days). Excessive clumping of cells can often be cured by trypsinizing the cells to disperse them. |
| Cryopreservation |
Complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
JE Becker |
