人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

• 0.005 mg/ml Insulin (Sigma I1882)
• 0.01 mg/ml Transferrin (Sigma T1147)
• 30nM Sodium selenite (final conc.) (Sigma S9133) 
• 10 nM Hydrocortisone (final conc.) (Sigma H0135)
• 10 nM beta-estradiol (final conc.) (Sigma E2557)  
• extra 2mM L-glutamine (for final conc. of 4.5 mM) (Sigma G5783) 
• 5% fetal bovine serum (ATCC 30-2020)

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.