| 產(chǎn)品名稱 |
NCI-H2073 [H2073] |
| 商品貨號(hào) |
B165308 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage 3A, adenocarcinoma; non-small cell lung cancer |
| Age |
47 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was established in November 1988. |
| Clinical Data |
47 years
Caucasian
female
The patient was a smoker.
Thirty pack years |
| Comments |
ATCC® CRL-5909™ and CRL-5918™ arose from the same donor (see Mayba O, 2014) |
| Complete Growth Medium |
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
- Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Subcultivation Ratio: 1:3 to 1:6.
- Place culture vessels in incubators at 37°C.
|
| Cryopreservation |
Freeze medium: culture medium, 85%; FBS,10%; DMSO,5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 12 THO1: 6,9.3 TPOX: 9 vWA: 17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1988 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Mayba O, et al. Integrative analysis of two cell lines derived from a non-small-lung cancer patient – A panomics approach. Pac Symp Biocomput 2014: 75-86. PubMed: 24297535
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