| 產(chǎn)品名稱 |
NCI-H676B [H676B] |
| 商品貨號(hào) |
B165358 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; Derived from metastatic site: pleural effusion |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
suspension, multicell aggregates
(gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures) |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenocarcinoma |
| Age |
63 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment. The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb). The line does not exhibit any gross structural DNA abnormalities. The cells produce mucin, and occasionally signet ring cells are formed. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This is a hypodiploid cell line; modal number = 43; range = 39 to 47. Marker chromosomes consisted of over 70% of the chromosomes in each cell complement. Among the identifiable markers were t(1q8q), der(3)t(3;?)(q29;?), t(2q7q), der(9)t(9;14)(p13;q11) and der(11)t(?q23;?). All markers had a single copy per cells. The N5, N9, N10, N17, N18, N20, N21 and the X were the only structurally normal chromosomes found. Except for the paired N5, all others had single copies per cell. |
| Derivation |
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment. |
| Clinical Data |
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment. male Caucasian 63 years |
| Genes Expressed |
mucin |
| Cellular Products |
mucin |
| Comments |
The NCI-H676B cell line was derived by A.F. Gazdar and H. Oie in 1984 from the pleural fluid of a patient with adenocarcinoma of the lung taken prior to treatment. The cells produce a abnormal size p53 mRNA (2.3 kb) as well as the normal size mRNA (2.8 kb). The line does not exhibit any gross structural DNA abnormalities. The cells produce mucin, and occasionally signet ring cells are formed. |
| Complete Growth Medium |
ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:- 0.02 mg/ml insulin
- 0.01 mg/ml transferrin
- 25 nM sodium selenite (final conc.)
- 50 nM Hydrocortisone (final conc.)
- 1 ng/ml Epidermal Growth Factor (do not filter)
- 0.01 mM ethanolamine (final conc.)
- 0.01 mM phosphorylethanolamine (final conc.)
- 100 pM triiodothyronine (final conc.)
- 0.5% (w/v) bovine serum albumin (final conc.)
- 10 mM HEPES
- 0.5 mM sodium pyruvate (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5mM)
|
| Subculturing |
Protocol: This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: culture medium, 82.5%; fetal bovine serum, 10%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X CSF1PO: 9 D13S317: 10 D16S539: 11,12 D5S818: 11 D7S820: 12 THO1: 9.3 TPOX: 8,11 vWA: 16,19 |
| Isoenzymes |
AK-1, 2 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 0 PGM1, 1-2 PGM3, 2 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1984 |
| References |
Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures
gland-like clusters in suspension; the viability of cells in clusters is better than the viability of the single cells in the cultures
|
