| 產(chǎn)品名稱(chēng) |
R24 |
| 商品貨號(hào) |
B165559 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the M-18 antigen system. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a human melanoma cell line (M-18 antigen system) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a human melanoma cell line (M-18 antigen system) |
| Comments |
Animals were immunized with the SK-MEL-28 human melanoma cell line. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with the M-18 antigen system. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 with 2.0 mM L-glutamine adjusted to contain 1.5 g/L
sodium bicarbonate, 4.5 g/L glucose, 10.0 mM HEPES, and 1.0 mM sodium pyruvate,
90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 90%; DMSO, 10% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG3 |
