| 產品名稱 |
RAW 8.1 |
| 商品貨號 |
B165582 |
| Organism |
Mus musculus, mouse |
| Tissue |
spleen |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
BALB/c |
| Applications |
RAW 8.1 was established from a tumor induced by Abelson murine leukemia virus. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
RAW 8.1 was established from a tumor induced by Abelson murine leukemia virus. |
| Comments |
RAW 8.1 was established from a tumor induced by Abelson murine leukemia virus. Growth of this line is inhibited by 5 mcg/ml LPS. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor |
P Ralph |
| Deposited As |
Mus musculus |
| References |
Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429
Ralph P, et al. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem. Biophys. Res. Commun. 61: 1268-1275, 1974. PubMed: 4616699
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