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RPC5.4

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編號:B165623

品牌:Mingzhoubio

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產品名稱	RPC5.4
商品貨號	B165623
Organism	Mus musculus, mouse
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	plasmacytoma; myeloma
Strain	BALB/c
Applications	The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma. The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine. Tested and found negative for ectromelia virus (mousepox).
Karyotype	modal number = 60
Derivation	The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma.
Antigen Expression	H-2d
Genes Expressed	immunoglobulin; monoclonal antibody,H-2d
Cellular Products	immunoglobulin; monoclonal antibody
Comments	The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma. The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype	IgG2a; kappa light chain
Name of Depositor	B Mohit
Deposited As	Mus musculus
References	Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030 Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Schwaber J. Human lymphocyte -- mouse myeloma somatic cell hybrids: selective hybrid formation. Somatic Cell Genet. 3: 295-302, 1977. PubMed: 305122

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