| 產品名稱 | RT4 |
|---|---|
| 商品貨號 | B165636 |
| Organism | Homo sapiens, human |
| Tissue | urinary bladder |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | transitional cell papilloma |
| Age | 63 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This cell line is a transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | modal number = 49; range = 42 to 55. The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases. |
| Images |  |
| Clinical Data | 63 years Caucasian male |
| Antigen Expression | Antigen expression: HLA A25(10), A3, B12, Cw3; Blood Type O |
| Tumorigenic | Yes |
| Effects | Yes, in cheek pouch of steroid-treated hamsters |
| Comments | A contamination with Mycoplasma orale was cured in October 1971. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8 D16S539: 9 D5S818: 11,12 D7S820: 9,12 THO1: 9,9.3 TPOX: 8,11 vWA: 14,17 |
| Isoenzymes | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor | C Rigby, LM Franks |
| Deposited As | Homo sapiens |
| References | O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125. O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572 Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035 Rigby CC, Franks LM. A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure. Br. J. Cancer 24: 746-754, 1970. PubMed: 5503601 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
