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X22

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編號:B165965

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產品名稱	X22
商品貨號	B165965
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody reacts with human, rodent and bovine clathrin, and is useful for immunofluorescence and immunoprecipitation applications.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Cellular Products	immunoglobulin; monoclonal antibody; against human clathrin heavy chain
Comments	Animals were immunized with human brain clathrin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with human, rodent and bovine clathrin, and is useful for immunofluorescence and immunoprecipitation applications.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15% This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
Subculturing	Cultures can be established by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. Maintain cell density between 10⁵ and 10⁶ viable cells/mL. Medium Renewal: Two to three times weekly
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG1
Name of Depositor	FM Brodsky
Deposited As	mouse (B cell); mouse (myeloma)
References	Brodsky FM. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J. Cell Biol. 101: 2047-2054, 1985. PubMed: 2415533 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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