| 產品名稱 |
YN1/1.7.4 |
| 商品貨號 |
B165989 |
| Organism |
Rattus norvegicus (B cell); Rattus norvegicus (myeloma), rat (B cell); rat (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes. |
| Derivation |
Spleen cells were fused with Y3 rat myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against murine ICAM-1 (ICAM 1, intercellular adhesion molecule) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against murine ICAM-1 (ICAM 1, intercellular adhesion molecule) |
| Comments |
Animals were immunized with NS-1 mouse myeloma cells. Spleen cells were fused with Y3 rat myeloma cells. The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes. The antibody partially inhibits Con A stimulation of spleen cells but does not inhibit LPS stimulation. The antibody strongly inhibits mixed lymphocyte responses (MLR). The ICAM-1 antigen is absent on most unstimulated thymocytes and spleen cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml. |
| Isotype |
IgG2b |
| Name of Depositor |
F Takei |
| Deposited As |
rat (B cell); rat (myeloma) |
| References |
Takei F. Inhibition of mixed lymphocyte response by a rat monoclonal antibody to a novel murine lymphocyte activation antigen (MALA-2). J. Immunol. 134: 1403-1407, 1985. PubMed: 2981916
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