Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Comments
Activities with high potential for aerosol production require Biosafety Level 3 facilities and practices. The inclusions are iodine stain negative (contain no glycogen). The TWAR strains of Chlamydia are named after TW183 and AR39. This material is cited in a U.S. and/or other Patent Application and may not be used to infringe the patent claims.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Disrupt cells in the inoculum by sonicating for 20 seconds at approximately 240W or by adding glass beads to the inoculum and vortex mixing. Remove medium and inoculate with disrupted material. Centrifuge at 2,300 - 2,400 x g at 20°C for 1 hour. End adsorption by adding agent growth medium.
Incubation: 3 days at 35°C in a humidified 5% CO2 atmosphere.
Throat of university student with acute pharyngitis, Seattle, WA, 1983
References
Grayston JT, et al. Chlamydia pnemoniae sp. nov. for Chlamydia sp. Strain TWAR. Int. J. Syst. Bacteriol. 39:88-90, 1989.
Kuo CC, Grayston JT. Chlamydia spp. strain TWAR: A newly recognized organism associated with atypical pneumonia and other respiratory infections. Clin. Microbiol. Newsletter 10: 137-140, 1988.
