Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Host strain E. coli JM103. Ten milliter of YT broth is inoculated with a single colony isolate. The overnight culture is diluted 1:10 into YT broth, and incubated for twelve hours and the supernatant is harvested.
Medium
ATCC® Medium 2975: M9 Minimal Agar + 40ug/ml Streptomycin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor
JC Grosch
References
Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580, 1983. PubMed: 6345791
