| 產(chǎn)品名稱 | alpha TC1 clone 6 |
|---|---|
| 商品貨號 | B171426 |
| Organism | Mus musculus, mouse |
| Tissue | pancreas, alpha cells |
| Cell Type | alpha cells |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent, with loosely attached clusters and some single cells in suspension |
| Biosafety Level | 2 [Cells contain Papovavirus (SV40)]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenoma |
| Applications | The alpha TC1 clone 6 cells are useful for studying many aspects of islet biology, including glucagon biosynthesis and cytokine sensitivity. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Alpha TC1 clone 6 is a pancreatic alpha cell line. It was cloned from the alpha TC1 cell line, which was derived from an adenoma created in transgenic mice expressing the SV40 large T antigen oncogene under the control of the rat preproglucagon promoter. |
| Receptor Expression | H-2b; H-2d, expressed |
| Genes Expressed | glucagon |
| Cellular Products | glucagon |
| Comments | The parental cell line is less differentiated and produces both glucagon and insulin. Two clonal cell lines, alpha TC1 clone 6 and alpha TC1 clone 9 (ATCC CRL-2350), are more differentiated than the parent line and produce only glucagon Ref   Hamaguchi K, Leiter EH. Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell lines. Viability, secretory function, and MHC antigen expression. Diabetes 39: 415-425, 1990. PubMed: 2108069. alpha TC1 clone 6 cells exhibit the most differentiated phenotype and express the highest levels of glucagon. RefHamaguchi K, Leiter EH. Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell lines. Viability, secretory function, and MHC antigen expression. Diabetes 39: 415-425, 1990. PubMed: 2108069 |
| Complete Growth Medium | The base medium for this cell line is Dulbecco's Modified Eagle's Medium, low glucose (Gibco Cat. No. 11885-084). To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
NOTE: Warm all solutions to 37.0°C prior to use
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended. Medium renewal: Every 2 to 3 days.
|
| Cryopreservation | Freeze medium: complete growth medium supplemented with an additional 40% heat-inactivated fetal bovine serum and 5% (v/v) DMSO |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 90%; carbon dioxide (CO2), 10% |
| Name of Depositor | EH Leiter |
| Year of Origin | 1988 |
| References | Powers AC, et al. Proglucagon processing similar to normal islets in pancreatic alpha-like cell line derived from transgenic mouse tumor. Diabetes 39: 406-414, 1990. PubMed: 2156740 Hamaguchi K, Leiter EH. Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell lines. Viability, secretory function, and MHC antigen expression. Diabetes 39: 415-425, 1990. PubMed: 2108069 Hamaguchi K, et al. Cellular Interaction Between Mouse Pancreatic alpha-Cell and beta-Cell Lines: Possible Contact-Dependent Inhibition of Insulin Secretion. Exp. Biol. Med. 228:1227-1233, 2003. PubMed: 14610265 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
