| 產(chǎn)品名稱 | hTERT-HPNE |
|---|---|
| 商品貨號 | B171429 |
| Organism | Homo sapiens, human |
| Tissue | Pancreas, duct |
| Cell Type | intermediary cells formed during acinar-to-ductal metaplasia |
| Product Format | frozen |
| Morphology | Epithelial-like |
| Culture Properties | Adherent |
| Biosafety Level | 2 [cells containing SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Normal |
| Age | 52 years |
| Gender | Male |
| Storage Conditions | Liquid nitrogen vapor phase |
| Karyotype | This is a pseudodiploid human cell line of male origin with a modal chromosome number of 46 and a low polyploidy rate. Approximately 50% of the cells contained a consistent derivative chromosome 21 with additional material at p12. |
| Images |  |
| Derivation | The hTERT-HPNE cell line was developed from human pancreatic duct by transduction with a retroviral expression vector (pBABEpuro) containing the hTERT gene. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings Ref |
| Comments | Exposing hTERT-HPNE cells to sodium butyrate and 5-aza-2'-deoxycytidine lead to the formation of pancreatic ductal cells marked by the expression of p-glycoprotein (multidrug resistance (MDR-1)), carbonic anhydrase II, and the cytokeratins 7, 8, and 19. hTERT-HPNE cells were found to have properties of the intermediary cells formed during acinar-to-ductal metaplasia, which included their undifferentiated phenotype, expression of Nestin and, evidence of active Notch signaling Ref |
| Complete Growth Medium | The base medium for this cell line is:
|
| Subculturing | Volumes used in this protocol are for 75 cm2. flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:8 - 1:12 twice weekly
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation | Freeze Medium: 95% FBS; 5% DMSO |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile | D5S818: 11 D13S317: 12,13 D7S820: 9,10 D16S539: 12,13 vWA: 17 Amelogenin: X,Y TPOX: 8,11 CSF1PO: 12 TH01: 8,9 |
| Population Doubling Level (PDL) | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor | M Ouellette |
| References | Lee KM. et al. Notch 2-positive progenitors with the intrinsic ability to give rise to pancreatic ductal cells. Lab. Investigation 85 (8): 1003-1012, 2005. Pubmed: 15924149 Lee KM. et al. Immortalization with telomerase of the Nestin-positive cells of the human pancreas. Biochem Biophys Res Commun. ; 301(4):1038-44 (2003). Pubmed: 12589817 Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
Lee KM. et al. Immortalization with telomerase of the Nestin-positive cells of the human pancreas. Biochem. Biophys. Res. Commun. 301(4):1038-1044, 2003. Pubmed: 12589817