| 產(chǎn)品名稱 |
pH5C |
| 商品貨號 |
B171513 |
| Designations |
pH5C |
| GenBank Number |
M57627
|
| Species |
Homo sapiens, human |
| Depositors |
DNAX Research Institute of Molecular and Cellular Biology, Inc., KW Moore, DNAX Research Institute of Molecular and Cellular Biology, Inc. |
| Applications |
in another host, produces protein interleukin 10 |
| Vector |
Construct size (kb): 0.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.580000042915344 Tissue: T-lymphocyte B21 cell line, activated Gene product: interleukin 10 [IL10] Target Gene: interleukin 10 |
| Insert Size (kb) |
1.580 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 1.8; BglII/HindIII--3.1, 1.15, 0.91; HindIII--3.2, 1.75. Contains the complete coding sequence. Contains 20 fewer bp in the 5' untranslated region than pH15C (ATCC 68192). The mobility of the protein produced by COS-7 transfectants is not affected by tunicamycin. The 3' untranslated region contains an Alu repeat. A 0.7 kb BglII/HindIII fragment from the insert is repeat-free. The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR). |
| References |
Brigino E, et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366
Mosmann TR, et al. Nucleic acids encoding cytokine synthesis inhibitory factor (interleukin-10). US Patent 5,231,012 dated Jul 27 1993
Vieira P, et al. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading fram BCRFI. Proc. Natl. Acad. Sci. USA 88: 1172-1176, 1991. PubMed: 1847510
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
