| Vector |
Construct size (kb): 4.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ATP1A2
Genomic copy number: unique
Gene name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q21-q23
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.7
Cross references: DNA Seq. Acc.: M78212; EST: EST01805; Primer: M13 Forward;
THC id: THC122363
DNA Seq. Acc.: M86176; EST: EST02708; Primer: M13 Reverse;
THC id: THC122363 Insert lengths(kb): 0.699999988079071 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide [ATP1A2] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 0.7; BamHI--4.0; PvuII--2.8, 1.2. EST01805 matches GenBank accession HUMATP1A2 with 99.64% identity over the sequence range: 1-278. EST02708 contains an Alu repeat element. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
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