| Vector Information |
Size (kb): 6.02 Vector: pRS415 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):LEU2,ampR Construct size (kb): 6.02 Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6 |
| Comments |
Restriction digests of the clone give the following sizes (kb): PvuII--5.55, 0.45; BglI--4.3, 1.7; ClaI--4.45, 1.55. A YC-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
