| Comments |
Approximate restriction sites are as follows: NarI-180, NcoI-220; SmaI-230, 670; PstI-410; KpnI-745; BglII-1000; PvuII-1880 bp. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.0; PstI/PvuII--2.3, 1.5, 0.5, 0.4, 0.2; PvuII/BglII--2.3, 1.2, 0.9, 0.4; HindIII--5.0; BamHI--5.0. This probe detects the following human genomic restriction fragments (kb): SfiI--150, 300, 550, 700; SalI--370, 730, 850, greater than 1000; SacII, PvuI, MluI--greater than 2000; SfiI/PvuI--150. Encodes entire coding sequence; about 190 bp 5' UT including an AUG 101 bp upstream followed by a 19 codon open reading frame; about 570 bp 3' UT with 3 potential polyadenylation sites. Cross-hybridizes with genes from hamster, mouse, rat, and pig. |
| References |
Yang-Feng TL, et al. Chromosomal organization of adrenergic receptor genes. Proc. Natl. Acad. Sci. USA 87: 1516-1520, 1990. PubMed: 2154750
Kobilka BK, et al. cDNA for the human beta2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor. Proc. Natl. Acad. Sci. USA 84: 46-50, 1987. PubMed: 3025863
Robert J Lefkowitz, personal communication
David M Allen, personal communication
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