| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--8.4 (doublet), 4.6, 4.2, 3.9 (doublet); EcoRI--8.4, 6.6, 5.2, 3.4, 2.3 (doublet), 1.9, 1.5; HindIII--11.0, 9.0, 7.4, 5.2, 3.4, 2.9, 2.1; BglI--8.4, 6.6, 6.2, 5.2, 3.9, 1.8, 1.6; SalI--30.0, 2.4; NotI--25.0, 8.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs are detected after competitive hybridization with total human DNA. Enzyme(s) not detecting polymorphism: TaqI, RsaI, BglII, PstI, PvuII. |
| References |
White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325
Bragg T, et al. Isolation and mapping of a polymorphic DNA sequence (cTB14.36) on chromosome 10 [D10S35]. Nucleic Acids Res. 16: 11390, 1988. PubMed: 2462728
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