| Insert |
DNA: cDNA Insert lengths(kb): 0.2029999941587448 Tissue: fetal muscle Gene product: myosin, light polypeptide 1, alkali; skeletal, fast [MYL1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.75; BamHI--3.2, PstI--3.2; HindIII--3.2; SmaI--3.2. An EcoRI digest analyzed on a 6% acrylamide gel revealed a band at 0.2 kb. This is a subclone of hMLC-1 which is specific to MLC1 RNA. Detects an mRNA of 1 kb in fast muscle fibers. pMLC1spec contains two inserts of 0.2 kb each. |
| References |
Seidel U, et al. Alkali myosin light chains in man are encoded by a multigene family that includes the adult skeletal muscle, the embryonic or atrial, and nonsarcomeric isoforms. Gene 66: 135-146, 1988. PubMed: 2458299
Seidel U, et al. The complete nucleotide sequences of cDNA clones coding for human myosin light chains 1 and 3. Nucleic Acids Res. 15: 4989, 1987. PubMed: 3601661
Hans H Arnold, personal communication
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