| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--13.0, 7.4 (doublet), 6.4, 6.2, 5.6, 1.7, 1.55, 0.94; BglII--17.0, 9.4, 6.4, 3.1, 2.9, 2.75, 2.2, 1.75, 1.45, 1.35, 1.25, 0.42; EcoRI--16.0, 11.0, 8.4, 8.0; HindIII--20.0; 11.5, 10.0, 4.0, 2.85, 1.2; SstI--12.0, 8.4, 6.4, 5.9, 5.6, 4.0, 3.4, 1.85, 1.2, 0.8, 0.6. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert contains part of intron 3' (between exon 2' and exon 2). |
| References |
Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859
Heisterkamp N, et al. The first BCR gene intron contains breakpoints in Philadelphia chromosome positive leukemia. Nucleic Acids Res. 16: 10069-10081, 1988. PubMed: 3194194
Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185
Bruce A Roe, personal communication
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