| 產品名稱 |
pAL-14 |
| 商品貨號 |
B172199 |
| Designations |
pAL-14 |
| Species |
Rattus norvegicus, rat |
| Depositors |
M Barbacid |
| Applications |
Two are copies of a HpaII/SacI fragment derived from 5' flanking sequences and used as a probe to detect the diagnostic MnlI RFLP. The HpaII/HpaII fragment is of unknown origin. This clone has been used to detect the MnlI RFLP indicative of a G to A mutation in codon 12 of the rat Ha-ras-1 locus induced by N-nitroso-N-methylurea. |
| Vector |
Construct size (kb): 3.099999904632568 |
| Insert |
DNA: genomic Insert lengths(kb): 0.3600000143051148 Tissue: mammary tumor Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS] |
| Insert Size (kb) |
0.360 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Contains three 120 bp fragments. Two are copies of a HpaII/SacI fragment derived from 5' flanking sequences and used as a probe to detect the diagnostic MnlI RFLP. The HpaII/HpaII fragment is of unknown origin. This clone has been used to detect the MnlI RFLP indicative of a G to A mutation in codon 12 of the rat Ha-ras-1 locus induced by N-nitroso-N-methylurea. The normal allele is 206 bp and the mutant is 280 bp. |
| References |
Zarbl H, et al. Direct mutagenesis of Ha-ras-1 oncogenes by N-nitroso-N-methylurea during initiation of mammary carcinogenesis in rats. Nature 315: 382-385, 1985. PubMed: 3923365
|
